Frontiers in Pharmacology-08 March 2019 新绿原酸绿原酸异绿原酸B

郑重说明:

我司所有产品仅供科研实验，不得用于注射、食用或其他用途

产品关键词：丹酚酸B 金丝桃苷人参皂苷rb1 雷公藤甲素

guanyuwomen关于德思特

联系方式

服务热线：

028-8500-3950

公司传真：	028-85589956
公司邮箱：	sales@tcmlmt.com
公司网址：	www.028desite.com
公司地址：	四川省成都市温江区海科路西段128号5栋

发表文献展示

当前位置:首页关于德思特 > 发表文献展示 >

时间：2020-09-03 浏览次数：分享到：

A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography
期刊名：Frontiers in Pharmacology
文献编号：DOI 10.3389/fphar.2019.00227
文献地址：https://www.frontiersin.org/articles/10.3389/fphar.2019.00227/full
发表日期：08 March 2019

A simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in Angelicae Pubescentis Radix (APR) by ultrahigh performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA). Diol was chosen as the dispersing sorbent and methanol solution was used as the elution solvent. The preparation procedures for the MSPD including the types of sorbents, mass ratio of matrix to sorbent, grinding time, type, concentration and volume of elution solvent were investigated. Under the optimal conditions, good recoveries of the 13 target compounds were obtained in the range of 94.8–107% (RSD < 3.22%). The limits of detection (LODs) and limits of quantitation (LOQs) were in the ranges of 0.08–0.12 μg mL-1 and 0.16–0.24 μg mL-1, respectively. Compared with the traditional method, it was a green and environmentally friendly technique. The results proved that the established method was successfully applied to the extraction and determination of 13 target bioactive compounds for quality control in APR.

neochlorogenic acid, chlorogenic acid, 4-dicaffeoylquinic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C,umbelliferae, columbianetin, imperatorin, osthole, isoimperatorin were obtained from Chengdu Desite Biological Technology Co., Ltd. (Chengdu, China).