德思特生物专注于中药(植物药)物质基础研究以及天然化合物、中药化学对照品实物生产供应


天然药物活性单体及中药标准品专业生产供应商

联系方式

028-85003950

联系方式

服务热线:

028-8500-3950

公司传真:028-85589956
公司邮箱:sales@tcmlmt.com
公司网址:www.028desite.com
公司地址:四川省成都市温江区海科路西段128号5栋


发表文献展示

当前位置:首页关于德思特 > 发表文献展示 >

Journal of Analytical Methods in Chemistry - 13 April 2020 桉油精 α-松油

时间:2020-09-03   浏览次数:  分享到:
Identification and Quality Evaluation of Raw and Processed Asarum Species Using Microscopy, DNA Barcoding, and Gas Chromatography-Mass Spectrometry
期刊名:Journal of Analytical Methods in Chemistry
文献编号:DOI 10.1155/2020/2690238
文献地址:https://www.hindawi.com/journals/jamc/2020/2690238/
发表日期:13 Apr 2020

Abstract
Asarum (Aristolochiaceae) is one of the common herbs used to relieve exterior syndromes. Some volatile components of Asarum which have toxic effect may cause adverse reactions such as headache, general tension, unconsciousness, and respiratory paralysis. Therefore, Asarum is normally processed to reduce such toxicity and adverse effects. The bioactive ingredients contained in different Asarum herbs vary significantly; this variation may be attributed to their differences in species, origins, or processing methods. In this study, 16 batches of Asarum herbs were collected, and their species were identified using DNA barcoding, which is a method for distinguishing plant species, coupled with microscopy. A gas chromatography-mass spectrometry (GC-MS) method for simultaneous determination of 10 compounds was established to evaluate the contents of raw and processed Asarum herbs. Multivariate analysis was then applied to compare different batches of herbs based on the GC-MS data. DNA barcoding identified the herbs as being derived from four sources, and herbs from different origins showed different microscopic features. The results demonstrated that most of the samples were clearly clustered into distinct groups that corresponded to species types. All raw and processed samples were classified by partial least squares discriminant analysis (PLS-DA) based on the 10 analyzed compounds. The findings suggested that safrole and methyleugenol with a variable importance in the project (VIP) > 1 are unique compounds that can be used to differentiate between Asarum species. Safrole, methyleugenol, and 2,6,6-trimethylcyclohepta-2,4-dien-1-one were identified as significant constituents, the presence of which can be used to differentiate between raw and processed Asarum samples. These results indicate that species and processing methods show important effects on the composition of Asarum herbs.

Chemicals. Reference standards of (1R)-(+)-alpha-pinene(+)-car-3-enecineole(−)-β-pinene, (−)-borneol2,6,6-trimethylcyclohepta-2,4-dien-1-oneα-terpineolestragolesafrole, and methyleugenol were purchased from Chengdu Desite Biological Technology Co. Ltd. (Sichuan, China)
 

热门新闻

Afrormosine CAS:550-79-8 中药对照品标准品

产品编码:DA0040产品名称:Afrormosine英文名:AfrormosineCAS登录...

查看更多

德思特
版权所有:成都德思特生物技术有限公司 Copyright © 2016   蜀ICP备16023840号-2
公司地址:四川省成都市温江区海科路西段128号5栋
公司电话:028-85003950

扫一扫关注德思特
   扫码关注公众号