Thrombin-based discovery strategy of bioactive-chemical quality marker combination for pollen of Typha orientalis by metabolomics coupled with chemometrics
期刊名：Phytomedicine 文献编号：DOI 10.1016/j.phymed.2020.153246 文献地址：https://www.sciencedirect.com/science/article/abs/pii/S0944711320300775?via%3Dihub 发表日期：14 May 2020 Abstract Background It is of utmost significance to choose the bioactive components as quality markers for ensuring the effectiveness of traditional Chinese medicine. Nonetheless, some markers are able to assess effectively the quality of TCM without considering the pharmacological mechanisms and intrinsic chemical complexities. Objective This underscores the need to discover new and efficient markers which can assess both quality and mechanism of action. Herein, a strategy of bioactive-chemical quality markers combination was proposed to improve the level of the quality control of TCM by metabolomics coupled with chemometrics.
Reference standards of typhaneoside, kaempferol-3-O-neohesperidoside, citric acid, linolenic acid, kaempferol-3-O-neohesperidoside, astragalin, naringenin, puerarin, and succinic acid with 98% purity on the basis of HPLC analysis were supplied by Chengdu DesiteBiological Technology Co. Ltd. (Sichuan, China)
